Graduate research using environmental DNA (eDNA) to monitor Spiny Lobster in Florida Bay

Hello, my name is Reneisha Sweet. I am a NOAA CCME-II EPP/MSI Graduate Scholar pursuing my master’s in biology with a marine and environmental science concentration at Jackson State University. I am an aspiring molecular ecologist and marine scientist interested in utilizing advanced molecular tools to monitor biodiversity and support marine conservation efforts. My thesis research focuses on developing probe-based quantitative PCR (qPCR) assays for the COI loci to enhance the detection and monitoring of Panulirus argus  (Caribbean spiny lobster). 

I had the incredible opportunity to dive into a 12-week NOAA Experiential Research and Training Opportunity (NERTO) Internship at the Atlantic Oceanographic and Meteorological Laboratory (AOML) in Miami, Florida, under the guidance of Drs. Katherine Silliman and Stephanie Roalses. I embarked on a project titled Spiny Lobster eDNA that combined cutting-edge molecular techniques with a passion for marine conservation. My project aimed to enhance our understanding of biodiversity in critical juvenile spiny lobster habitats while improving tools for species-specific monitoring.

The experience wasn’t confined to the lab; I had the chance to join fieldwork expeditions in the Florida Keys alongside the Florida Fish and Wildlife Conservation Commission. Collecting environmental DNA samples and other data on Caribbean spiny lobsters in such a breathtaking setting was exhilarating and humbling, underscoring the importance of preserving these unique ecosystems. 

Beyond my hands-on work, I participated in weekly journal club meetings with NOAA scientists and fellow interns. These sessions became a creative and collaborative hub, sparking discussions on the latest research, fostering new ideas, and providing constructive feedback that pushed my scientific thinking. This dynamic environment expanded my technical and analytical skills and fueled my enthusiasm for marine science.

My NERTO project seamlessly connected to my master’s research, allowing me to further develop my molecular and bioinformatics expertise. I also began exploring how environmental factors like temperature and habitat characteristics might shape the biodiversity and health of P. argus habitats. These experiences solidified my commitment to using molecular tools to tackle ecological challenges and guide sustainable resource management.

This internship was more than a learning experience. It was a transformative journey that deepened my appreciation for the delicate balance of marine ecosystems and reaffirmed my drive to contribute to their conservation through science. The mentorship and support I received during my NERTO were instrumental in my growth as a scientist. This experience strengthened my aspiration to pursue a Ph.D., where I aim to integrate molecular biology, ecological modeling, and conservation strategies to enhance the sustainable management of marine resources. I am deeply grateful for the opportunity to contribute to NOAA’s mission and to further my journey as a marine scientist.

NOTE: Reneisha Sweet’s NERTO internship was made possible by the National Oceanic and Atmospheric Administration (NOAA) Office of Education, Educational Partnership Program with Minority-Serving Institutions award #NA21SEC4810004 (NOAA Center for Coastal and Marine Ecosystems-II). The contents of this post are solely the responsibility of the award recipient and do not necessarily represent the official views of the U.S. Department of Commerce, NOAA.